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NT-proBNP ELISA
|
貨號: |
SK-1204 |
|
方法學: |
Sandwich ELISA, HRP/TMB, 12x8-well strips |
|
樣本類型: |
Serum and EDTA plasma |
|
標準品范圍: |
0-640 pmol/l (5 serum based standards) |
|
標準品濃度: |
0/10/40/160/640 pmol/l |
|
質控品: |
1 serum based control |
|
樣本量: |
50 μl / well |
|
孵育時間: |
3 h / 30 min |
|
單位換算: |
1 pg/ml = 0.118 pmol/l (MW: 8.475 kDa) |
靈敏度:
LOD: 3 pmol/l (0 pmol/l + 3 SD)
精密度：
Intra-assay (n=3) ≤ 3%, Inter-assay (n=8) ≤ 7%
回收率：
The mean recovery of recombinant NT-proBNP in serum samples (n=4) is 104%, in EDTA plasma samples (n=4) is 94%.
線性： Dilution linearity for serum samples (n=3) is between 79-86% for endogenous NT-proBNP (1-76) and 112-119% for recombinant NT-proBNP (1-76).
Dilution linearity for EDTA plasma samples (n=2) is between 83-84% for endogenous NT-proBNP (1-76) and 83-86% for recombinant NT-proBNP (1-76).
交叉反應:
The assay works with human samples only.
參考范圍（健康人群）：
Median (serum, n=70): 5.8 pmol/l.
Median (EDTA plasma, n=28): 5.6 pmol/l.
推薦實驗室建立自己的參考值。
僅供科研使用。
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