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貨號: |
BI-20892 |
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方法學: |
Sandwich ELISA, HRP/TMB, 12x8-well strips |
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樣本類型: |
Serum, plasma (EDTA, heparin), cell culture supernatant, urine |
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標準品范圍: |
0-10 nmol/l (6 serum based standards) |
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標準品濃度: |
0/0.63/1.25/2.5/5/10 nmol/l |
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質控品: |
1 serum based control |
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樣本量: |
10 μl / well |
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孵育時間: |
3 h / 30 min |
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單位換算: |
1 ng/ml = 0.079 nmol/l (MW: 12.7 kDa) |
靈敏度：
LOD: 0.05 nmol/l (0 pmol/l + 3 SD)
精密度：
Intra-assay (n=10) ≤ 2%, Inter-assay (n=5) ≤ 4%
回收率：
The mean recovery of recombinant proANP (1-98) in human plasma samples (n=4) is 87%.
線性：
Dilution linearity of endogenous proANP (1-98) with assay buffer is:
Mean for 1+1 dilution = 90%
Mean for 1+3 dilution = 87%
Mean for 1+7 dilution = 79%
交叉反應：
The assay cross-reacts with rat or mouse samples.
proANP(1-30) <1%, proANP(31-67) <1%, proANP(79- 98) <1%, alpha ANP( 99-126)<1%, proBNP(8-29)<1%, proBNP(32-57) <1%, proCNP(1-19) <1%, proCNP(30-50) <1%, proCNP(51-97) <1%.
參考范圍（健康人群）：
Median (plasma, n=53): 1.45 nmol/l.
推薦實驗室建立自己的參考值。
僅供科研使用。
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