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【產(chǎn)品說明】 
失活的牙質(zhì)片層用作骨重吸收培養(yǎng)基。這些片層以5 mm直徑的牙質(zhì)薄片形式提供（正常厚度為0.3 mm）。每批培養(yǎng)基均經(jīng)過檢測以確保被有功能的破骨細(xì)胞重吸收。另可提供預(yù)重吸收的樣品片層以便于體外重吸收功能鑒定。僅供科研使用。生產(chǎn)廠商：艾狄斯

【簡介】 
牙質(zhì)片層以50片一包形式供應(yīng)，包裝于已消毒的旋轉(zhuǎn)蓋封容器內(nèi)。該片層通過紫外線殺菌并且只能在無菌環(huán)境（如層流柜）中打開。

【儲存】 
該產(chǎn)品可在室溫下儲存。如果容器不打開且片層保持干燥，在保質(zhì)期內(nèi)不會出現(xiàn)變質(zhì)。

【應(yīng)用】
該產(chǎn)品被設(shè)計利用“骨切片檢測”以實現(xiàn)體外骨重吸收量化(1,2)，在“骨切片檢測”中，培養(yǎng)的破骨細(xì)胞在礦化的底層挖掘出真正的骨重吸收間隙。從該檢測的第一個表述開始，在過去的16年間(1,2)，該檢測已被廣泛地應(yīng)用于研究許多因素對骨重吸收的影響且是當(dāng)今體外骨重吸收研究使用最廣泛的方法(3-8)。

骨切片檢測能與從實驗動物（包括小鼠、大鼠、雞、兔和貓）的骨分離的破骨細(xì)胞同時使用。它已被廣泛用于研究通過直接分離于骨或培養(yǎng)自骨髓培養(yǎng)基的人破骨細(xì)胞重吸收 (4)。

該領(lǐng)域當(dāng)今主要優(yōu)勢之一是通過RANK配基的增加從外周紅細(xì)胞誘導(dǎo)破骨細(xì)胞形成的技術(shù)的發(fā)展(9)。利用這項技術(shù)，大量功能性破骨細(xì)胞能被激活，被激活的功能性破骨細(xì)胞將在包括骨位點重吸收的牙質(zhì)片層在內(nèi)的礦化底層挖掘出重吸收間隙。重吸收很容易被反射光顯微鏡方法而量化(3,4,6,8)。

【參考文獻(xiàn)】 
1. Boyde A, Ali N N and Jones S J (1984) Resorption of dentine by isolated osteoclasts in vitro. Br. Dent. J. 156:216-220.
2. Chambers T J, Revell P A, Fuller K and Athanasou N (1984) Resorption of bone by isolated rabbit osteoclasts. J. Cell Sci. 55:383-399.
3. Walsh C A, Beresford J N, Birch M A, Boothroyd B and Gallagher J A (1991) Application of reflected light microscopy to identify and quantitate resorption by isolated osteoclasts. J. Bone Miner. Res. 6:661-671.
4. Walsh C A, Carron J A and Gallagher J A (1996) The isolation of osteoclasts from human giant cell tumours and long-term marrow cultures. In 'Human Cell Culture Protocols' Ed. G.E. Jones, Humana Press Inc. pp 233-262.
5. Arnett T R and Dempster D W (1987) A comparative study of disaggregated chick and rat osteoclasts in vitro: Effects of calcitonin and prostaglandins. Endocrinology 120:602-608.
6. Sato M and Grasser W (1990) Effects of bisphosphonates on isolated rat osteoclasts as examined by reflected light microscopy. J. Bone Miner. Res. 5:31-40.
7. Boyde A (1991) Pitfalls in pit measurement. Calcif. Tissue Int. 49:65-70.
8. Tamura T, Takahashi N, Akatsu T, Sasaki T, Udagawa N, Tanaka S and Suda T (1993) New resorption assay with mouse osteoclast-like multinucleated cells formed in vitro. J. Bone Miner. Res. 8:953-960.
9. Itonaga I, Sabokbar A, Neale S D, and Athanasou N A (1999) 1, 25 Dihydroxyvitamin D3 and prostaglandin E3 act directly on circulating human osteoclast precursors. Biochem. Biophys. Res. Comm. 264:590-595.