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Clostridium difficile antigen GDH

Description

CerTest Clostridium difficile antigen GDH one step card test is a coloured chromatographic immunoassay for the qualitative detection of Clostridium difficile Glutamate Dehydrogenase (GDH) in stool samples.

CerTest Clostridium difficile antigen GDH card test offers a simple and highly sensitive screening assay to make a presumptive diagnosis of Clostridium difficile infection.

Specifications

Method: Lateral Flow
Time to result: 10 minutes
Specimen Type: Faecal
Storage up to: 2 Years

About

Clostridium difficile (C. difficile), a Gram-positive spore bearing anaerobic bacterium is the major aetiological agent of diarrhoea and colitis associated with antibiotics.

C. difficile is the most common cause of health care-associated diarrhoea in developed countries and is a major source of nosocomial morbidity and mortality worldwide.

Disease due to C. difficile develops when the organism is allowed to proliferate in the colon, most commonly after antibiotic use has eliminated competing flora. C. difficile can release two high-molecular-weight toxins, toxin A and toxin B, which are responsible for the clinical manifestations, which range from mild, self-limited watery diarrhoea to fulminant pseudomembranous colitis, toxic megacolon and death.

Clostridium difficile Glutamate Dehydrogenase (GDH) is an enzyme produced in large quantities by all toxigenic and non-toxigenic strains, making it an excellent marker for the organism. The toxigenic culture (TC) is used as the gold standard technique to determine Clostridium difficile infection. This method consists in culture and isolation of C. difficile from faeces, followed by toxin testing of the isolate, a labour-intensive assay to obtain a result.

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